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ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. A positive spot test shows agglutination of horse erythrocytes when added to patient serum previously absorbed with guinea pig kidney, but not when added to patient serum previously absorbed with beef erythrocyte stroma. The test is generally simple, sensitive, and specific, but false negative are common in young children with mononucleosis.
The most common rapid slide test (MONOSPOT©) for infectious mononucleosis employs:

A) Sheep erythrocytes
B) Intact beef erythrocytes
C) None of the above
D) Horse erythrocytes

2. Using the formula on the right,

Cells/µL = 370 x 100 / 18 x 0.1
Cells/µL = 37000 / 1.8
Cells/µL = 20556 or 2.06 x 104
A sample of cerebrospinal fluid is diluted 1:100; the standard 9 squares of a hemocytometer are counted on each side for a total of 18 large squares.
Side 1-- 186 nucleated cells counted
Side 2-- 184 nucleated cells counted
total nucleated cells = 370
Using the standard hemocytometer formula shown on the right, what is the nucleated cell count per microliter (µL)?

A) 9.25 x 10^4
B) 2.06 x 10^4
C) 4.62 x 10^4
D) 1.03 x 10^4

3. Once substance H is developed, the addition of the sugar N-acetylgalactosamine to the terminal position of the chain gives the molecule "A" antigenic activity.
Which specific terminal sugar causes a red cell to have A antigenic activity?

A) Glucose
B) Galactose
C) N-acetylgalactosamine
D) Galactose plus glucose

4. In combined fiscal years 2005 through 2009, transfusion-related acute lung injury (TRALI) caused the higest number of reported fatalities (48%), followed by hemolytic transfusion reactions (26%) due to non-ABO (16%) and ABO (10%) incompatibilities. Complications of microbial infection, transfusion-associated circulatory overload (TACO), and anaphylactic reactions each accounted for a smaller number of reported fatalities.

A) Transfusion-associated graft versus host disea
B) Anaphylactic reactions
C) Febrile non-hemolytic reactions
D) Transfusion-related acute lung injury (TRALI)

5. Troponin is a very specific biomarker that is released during cardiac injury or stress. CK is found not only in cardiac tissue, but also in muscle and brain tissue. LD levels can be elevated in cardiac events, tissue breakdown, and hemolysis. Myoglobin is elevated when muscle tissue is damaged and is not specific for the heart muscle.
Chemistry
Which one of the following is the MOST specific biochemical marker of myocardial infarction?

A) myoglobin
B) Troponin
C) CK
D) LD

Solutions: